POLYPHASIC CHARACTERIZATION OF Bacillus STRAINS ISOLATED FROM MAIZE

Authors

  • ELIANE APARECIDA GOMES EMBRAPA MILHO E SORGO https://orcid.org/0000-0001-8488-3936
  • UBIRACI GOMES DE PAULA LANA EMBRAPA MILHO E SORGO
  • MARINA ELEUTÉRIO GÓI DE MORAES RODRIGUES CENTRO UNIVERSITÁRIO DE SETE LAGOAS - UNIFEMM
  • SYLVIA MORAIS DE SOUSA EMBRAPA MILHO E SORGO
  • CHRISTIANE ABREU DE OLIVEIRA EMBRAPA MILHO E SORGO
  • AMANDA NAYÊ GUIMARÃES TAVARES EMBRAPA MILHO E SORGO

DOI:

https://doi.org/10.18512/rbms2020v19e1190

Keywords:

Bacillus, molecular fingerprinting, plant growth-promoting, Zea mays L.

Abstract

The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subitilis (B2082 and B2084) using 16S rRNA gene sequencing and MALDI-TOF mass spectrometry. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants production quality control, strains traceability and to detail PGPB colonization.

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Published

2020-10-17

How to Cite

GOMES, E. A., PAULA LANA, U. G. D., RODRIGUES, M. E. G. D. M., DE SOUSA, S. M., OLIVEIRA, C. A. D., & GUIMARÃES TAVARES, A. N. (2020). POLYPHASIC CHARACTERIZATION OF Bacillus STRAINS ISOLATED FROM MAIZE. REVISTA BRASILEIRA DE MILHO E SORGO, 19, 18. https://doi.org/10.18512/rbms2020v19e1190

Issue

Vol. 19 (2020)

Section

Scientific Paper

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