CARACTERÍSTICAS CULTURAIS E PATOLÓGICAS DE Bipolaris maydis EM DIFERENTES MEIOS DE CULTURA
DOI:
https://doi.org/10.18512/1980-6477/rbms.v15n3p461-469Keywords:
Zea mayss L., Crescimento micelial, Helmintosporiose, Bipolaris maydis, Milho pipoca.Abstract
RESUMO A mancha de Bipolaris, causada por Bipolaris maydis, é uma das principais doenças foliares de Zea mays, apresentando um considerável potencial de dano, notadamente, na cultura do milho-pipoca, embora trabalhos sobre este patossistema ainda sejam escassos. Neste trabalho, avaliaram-se as influências de diferentes meios de cultura sobre o crescimento micelial, esporulação e a infectividade de inóculo conidial de B. maydis em folhas da cultivar suscetível Beija-flor, de milho-pipoca. Para tal, instalaram-se experimentos em laboratório e em casa de vegetação em blocos casualizados com quatro repetições. Em laboratório, avaliaram-se o crescimento micelial e a esporulação de B. maydis em diferentes meios de culturas (Batata Dextrose Ágar, Feijão Ágar, Água de Coco Ágar, Arroz Polido Ágar, Folha de Milho Ágar e Lactose Caseína Ágar). Em casa de vegetação, avaliaram-se o número de lesões por folha e a incidência de doença na planta suscetível inoculada com uma suspensão de 104 conídios/mL. O meio de Arroz Polido Ágar foi o que mais promoveu o crescimento micelial do fungo, porém não favoreceu a esporulação. Para esporulação, destacaram-se os meios de cultura Batata Dextrose Ágar e Feijão Ágar. Maior número de folhas com sintoma da doença e maior número de lesões por folha foram obtidos com o inóculo produzido no meio Feijão Ágar.
Palavras-Chave: Zea mays L., Crescimento micelial, Mancha de Bipolaris, Esporulação, Milho-pipoca.
CULTURAL AND PATHOLOGICAL FEATURES OF Bipolaris maydis IN DIFFERENT CULTURE MEDIA
ABSTRACT. The Southern corn leaf blight, caused by Bipolaris maydis, is a major foliar disease of corn, presenting considerable potential of damage, especially in Zea mays, although studies with this pathosystem are still scarce. In this paper, the effect of different culture media was evaluated on mycelial sporulation and infectivity of conidial inoculum of B. maydis on leaves of the susceptible cultivar Beija-Flor, of corn-popcorn. Experiments in laboratory and greenhouse were installed in a randomized blocks design with four replications. The mycelial growth and sporulation of B. maydis on different culture media (Potato Dextrose Agar, Bean Agar, Coconut Water Agar, Rice Polished Agar, Corn Leaf Agar Lactose Casein Agar) were evaluated in the laboratory. In the greenhouse it was evaluated the number of lesions per leaf and the incidence of disease in susceptible plants inoculated with a suspension of 104 conidia/ml. The culture medium Polished Rice Agar promoted the highest mycelial growth, but did not promote sporulation. Sporulation was higher on culture media Potato dextrose Agar and Agar Bean. A greater number of leaves with symptoms of the disease and a higher number of lesions per leaf were obtained with the inoculum produced in the culture medium Bean Agar.
Keywords: Zea mays L, Mycelial growth, Southern corn leaf bligh, Sporulation, Popcorn.
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